The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. Though the life-saving compound was initially found in the bark, its presence was later confirmed in every part of the tree excluding the fruit.1 Paclitaxel, is a member of the taxane family and has been associated with the highly toxic nature of the Taxus genus.2 Paclitaxel was discovered to inhibit mitosis in tumor cells and other fast growing cells through the binding of microtubules.3 This behavior led to its use as a tumor suppression agent. The isolation of paclitaxel from its major impurities is shown below with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
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Pick Your Poison. Isolation of Paclitaxel.
[fa icon="calendar'] October 29, 2021 / by Adam L. Moore, PhD
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Saccharide Identification in Honey by Cation Exchange HPLC (HC-75 Pb2+)
[fa icon="calendar'] November 25, 2020 / by Adam L. Moore, PhD
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Honey has been acknowledged for its medicinal and healing qualities for some time. Its ubiquity of the sticky golden yellow liquid spans time and continents. The substance has been used for treatments of skin disease, cancer, heart diseases, neurological degeneration, and wound healing and is considered anti-inflammatory and antimicrobial.1 These attributes have been attributed to the minor constituents found within, consisting of phenolics, flavonoids, flavones, and flavonols.2
Benzodiazepene Metabolites by Reversed-Phase HPLC (PRP-1)
[fa icon="calendar'] November 25, 2020 / by Adam L. Moore, PhD
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Benzodiazepines were first discovered in the 1960’s and reached a peak around 1970 with diazepam (Valium). During that time the drug was prescribed for any kind of antihypertensive, analgesic, or psychotropic medications1. The overall enthusiasm for the benefits of this class of drug has made a growing trend of addiction more difficult to treat and diagnose.
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